禽肿瘤病毒基因组由RNA指导的DNA聚合酶进行体外转录：对在重建酶促反应中合成的DNA转录本的分析
In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions.
作者信息
Collett M S, Faras A J
出版信息
J Virol. 1977 Apr;22(1):86-96. doi: 10.1128/JVI.22.1.86-96.1977.
Abstract
We have analyzed the DNA products synthesized in vitro in reconstructed reactions containing purified avian oncornavirus genome RNA and RNA-directed DNA polymerase. The results of these studies indicate that: (i) the initial DNA product synthesized on either 70S RNA or reconstituted 35S RNA-tRNAtrp template - primer complexes in the presence of low concentrations of deoxynucleoside triphosphates consists of several discrete size classes, none of which exceed 200 nucleotides in length; (ii) large DNA transcripts (about 2,000 nucleotides) can be synthesized on both 70S RNA and the 35S RNA - tRNAtrp complex by increasing the deoxynucleoside triphosphate concentration; and (iii) DNA synthesized by detergent-disrupted virus is considerably longer than DNA synthesized in reconstructed reactions.
摘要
我们分析了在含有纯化禽肿瘤病毒基因组RNA和RNA指导的DNA聚合酶的体外重建反应中合成的DNA产物。这些研究结果表明：（i）在低浓度脱氧核苷三磷酸存在下，在70S RNA或重组的35S RNA-色氨酸转运RNA模板-引物复合物上合成的初始DNA产物由几个离散的大小类别组成，其长度均不超过200个核苷酸；（ii）通过增加脱氧核苷三磷酸浓度，可以在70S RNA和35S RNA-色氨酸转运RNA复合物上合成大的DNA转录本（约2000个核苷酸）；（iii）由去污剂破坏的病毒合成的DNA比在重建反应中合成的DNA长得多。
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本文引用的文献
1
Iodination of nucleic acids in vitro.核酸的体外碘化
Biochemistry. 1971 May 25;10(11):1993-2000. doi: 10.1021/bi00787a005.
2
Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.由埃及嗜血杆菌的一种限制酶——核酸内切酶Z产生的φX174脱氧核糖核酸的特定片段。
J Virol. 1972 Jul;10(1):42-50. doi: 10.1128/JVI.10.1.42-50.1972.
3
Hemophilus aegyptius restriction edonuclease cleavage map of the simian virus 40 genome and its colinear relation with the hemophilus influenzae cleavage map of SV40.埃及嗜血杆菌对猴病毒40基因组的限制性核酸内切酶切割图谱及其与流感嗜血杆菌对SV40切割图谱的共线性关系。
J Mol Biol. 1974 Sep 5;88(1):105-23. doi: 10.1016/0022-2836(74)90297-6.
4
Characterization of the low-molecular-weight RNAs associated with the 70S RNA of Rous sarcoma virus.与劳氏肉瘤病毒70S RNA相关的低分子量RNA的特性分析。
J Virol. 1973 Aug;12(2):334-42. doi: 10.1128/JVI.12.2.334-342.1973.
5
Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species.禽白血病和肉瘤病毒RNA在甲酰胺中的凝胶电泳：与其他病毒和细胞RNA种类的比较
J Virol. 1973 Sep;12(3):594-9. doi: 10.1128/JVI.12.3.594-599.1973.
6
Ribonucleases of Rous sarcoma virus.劳氏肉瘤病毒的核糖核酸酶
Virology. 1973 Feb;51(2):287-96. doi: 10.1016/0042-6822(73)90429-7.
7
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Biochemistry. 1973 Jan 30;12(3):460-7. doi: 10.1021/bi00727a016.
8
Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus.与劳氏肉瘤病毒相关的脱氧核糖核酸聚合酶的纯化与特性分析
Biochemistry. 1972 Jun 6;11(12):2334-42. doi: 10.1021/bi00762a020.
9
RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。II. 反应中RNA的直接导向作用。
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J Virol. 1971 Jul;8(1):17-27. doi: 10.1128/JVI.8.1.17-27.1971.
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