Azmitia E C, Algeri S, Costa E
Science. 1970 Jul 10;169(3941):201-3. doi: 10.1126/science.169.3941.201.
Rats were adrenalectomized 10 days before we estimated in vivo the conversion index of (3)H-tryptophan into radioactive serotonin in brainstem and telediencephalon. We found that the conversion index in the brainstem of adrenalectomized rats is smaller than in the same area of sham-operated rats. Conversely, the conversion index in the telediencephalon was similar in the two groups of rats. The serotonin concentrations were unchanged by adrenalectomy, which suggests that in brainstem the decrease of tryptophan hydroxylase is reflected by the conversion index estimation and not by measurement of serotonin steady-state concentrations.
在我们对大鼠脑干和端脑内（3）H-色氨酸转化为放射性5-羟色胺的转化指数进行体内评估前10天，将大鼠肾上腺切除。我们发现，肾上腺切除大鼠脑干的转化指数小于假手术大鼠相同区域的转化指数。相反，两组大鼠端脑的转化指数相似。肾上腺切除对5-羟色胺浓度无影响，这表明在脑干中，色氨酸羟化酶的减少通过转化指数评估反映出来，而非通过5-羟色胺稳态浓度的测量反映。
