秋水仙碱与鸡坐骨神经轴浆蛋白的体外结合
The binding in vitro of colchicine to axoplasmic proteins from chicken sciatic nerve.
作者信息
James K A, Austin L
出版信息
Biochem J. 1970 May;117(4):773-7. doi: 10.1042/bj1170773.
Abstract
- Axoplasmic proteins were fractionated by means of Sephadex G-200 chromatography followed by isoelectric focusing. Nine groups of proteins were separated. 2. The binding of colchicine to these groups of proteins was examined and it appeared to associate most strongly with one protein group, of pI value 4.9-5.0, which is the major (14)C-labelled component of slow-transport protein. 3. Other fractions also bind colchicine. It is not clear whether these are separate proteins or subunits of the major colchicine-binding fraction.
摘要
- 通过葡聚糖凝胶G - 200色谱法随后进行等电聚焦对轴浆蛋白进行分级分离。分离出了九组蛋白。2. 检测了秋水仙碱与这些蛋白组的结合情况，结果显示它与一组pI值为4.9 - 5.0的蛋白结合最为强烈，该组蛋白是慢运输蛋白的主要（14）C标记成分。3. 其他组分也能结合秋水仙碱。尚不清楚这些是单独的蛋白质还是主要秋水仙碱结合组分的亚基。
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