大鼠膀胱上皮腔面质膜的分离，以及在负染整装标本和切片膜中均出现亚基的六边形晶格。
Isolation of the plasma membrane of the luminal surface of rat bladder epithelium, and the occurrence of a hexagonal lattice of subunits both in negatively stained whole mounts and in sectioned membranes.
作者信息
Hicks R M, Ketterer B
出版信息
J Cell Biol. 1970 Jun;45(3):542-53. doi: 10.1083/jcb.45.3.542.
Abstract
A method of isolating the thick luminal membrane from homogenates of bladder epithelium is described, which entails pretreatment of the epithelium with fluorescein mercuric acetate and centrifugation of the homogenate on sucrose density gradients. A hexagonal array of hexamers is illustrated by negative contrast staining in whole mounts of the isolated thick membrane. Subunits are also shown in tangential sections of this thick membrane, in fixed, embedded bladder epithelium. The significance of the subunits is discussed in the context of membrane structure and permeability.
摘要
本文描述了一种从膀胱上皮匀浆中分离厚腔膜的方法，该方法需要用醋酸汞荧光素对上皮进行预处理，并将匀浆在蔗糖密度梯度上离心。通过对分离出的厚膜整装片进行负染，显示出六聚体的六边形阵列。在固定、包埋的膀胱上皮的该厚膜的切向切片中也显示出亚基。在膜结构和通透性的背景下讨论了亚基的意义。
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