Hulshoff A, Perrin J H
J Chromatogr. 1976 May 12;120(1):65-80. doi: 10.1016/s0021-9673(01)98998-8.
A reversed-phase thin-layer chromatographic method has been developed for the determination of partition coefficients. A support phase has been chosen, following investigation of the lack of adsorptive properties, which has a minimal effect on the pH of the buffer system. A stationary phase has been chosen to give deltaRm values of the same magnitude as Hansch pi values for a series of phenothiazines. The method can be applied to molecules of a wide range of lipophilicity following preliminary investigations of suitable phase-volume ratios and of the pH and composition of the binary mobile phase, providing adsorption on the support phase is excluded.
已开发出一种反相薄层色谱法用于测定分配系数。在研究了缺乏吸附特性后，选择了一种对缓冲系统的pH值影响最小的支持相。选择了一种固定相，以使一系列吩噻嗪的δRm值与Hansch π值具有相同的量级。在对合适的相比以及二元流动相的pH值和组成进行初步研究后，该方法可应用于广泛亲脂性的分子，前提是排除在支持相上的吸附。
