Hulshoff A, Perrin J H
J Chromatogr. 1976 Dec 22;129:249-62. doi: 10.1016/s0021-9673(00)87783-3.
A reversed-phase thin-layer chromatographic technique was used for the characterization of 26 phenothiazine drugs. With two chromatographic systems having the sam stationary phase and phase volume ratio, but mobile phases of different pH, all but two of the compounds could be identified. Rf values in the different systems were standardized by applying a reference compound to the plates next to each compound under investigation; the corrected Rf values were calculated from the differences in the Rm values of the compounds and the reference compound, and the theoretical Rm value of the reference. It was shown that Rf values for different chromatographic systems with the same stationary phase could be predicted with reasonable accuracy. The pH of the mobile phase, for which a maximum difference in Rf values was obtained for pairs of compounds, could also be calculated and corresponded well with the observed values.
采用反相薄层色谱技术对26种吩噻嗪类药物进行表征。使用两个具有相同固定相和相比，但流动相pH不同的色谱系统，除两种化合物外，其余所有化合物均可被鉴定。通过将参考化合物点在待研究的每种化合物旁边的板上，对不同系统中的比移值进行标准化；校正后的比移值由化合物和参考化合物的比移值之差以及参考化合物的理论比移值计算得出。结果表明，对于具有相同固定相的不同色谱系统，其比移值能够以合理的准确度进行预测。还可以计算出化合物对的比移值差异最大时流动相的pH，该值与观察值吻合良好。
