甲基纤维素和其他聚合物对承受层流剪切应力的昆虫细胞的保护作用。

Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress.

作者信息

Goldblum S, Bae Y K, Hink W F, Chalmers J

机构信息

Department of Chemical Engineering, Ohio State University, Columbus 43210.

出版信息

Biotechnol Prog. 1990 Sep-Oct;6(5):383-90. doi: 10.1021/bp00005a011.

DOI:10.1021/bp00005a011

PMID:1370016

Abstract

The relative sensitivity of two insect cell lines to laminar shear stress was determined, and the protective effect of polymers added to the growth media of two insect cell lines, Trichoplusia ni (TN-368) and Spodoptera frugiperda (SF-9), was evaluated. TN-368 and SF-9 cells were found to be equally sensitive to laminar shear stress. Methylcellulose [0.5% (w/v) Dow E4M Methocel] and dextran [4.5% (w/v)] increased the resistance of suspended cells to lysis due to laminar shear stress by factors of up to 76 and 28, respectively, compared to cells in media without additives. It was observed that the protective effect of Pluronic F-68 was concentration-dependent: 0.2% and 0.3% (w/v) F-68 increased the resistance of SF-9 cells to shear stress by factors of 15 and 42, respectively. However, increasing the concentration to 0.5% did not significantly increase the cells' resistance compared to 0.3% (w/v). F-68 at 0.2% only increased the resistance of TN-368 cells by a factor of 6. It is believed that the protection is a result of the polymer adsorbing to the cell membrane. None of the polymer additives tested had a significant effect on SF-9 or TN-368 growth rate.

摘要

测定了两种昆虫细胞系对层流剪切应力的相对敏感性，并评估了添加到两种昆虫细胞系（粉纹夜蛾（TN - 368）和草地贪夜蛾（SF - 9））生长培养基中的聚合物的保护作用。发现TN - 368和SF - 9细胞对层流剪切应力同样敏感。与不含添加剂的培养基中的细胞相比，甲基纤维素[0.5%（w/v）陶氏E4M甲基纤维素]和葡聚糖[4.5%（w/v）]分别使悬浮细胞对因层流剪切应力导致的裂解的抗性提高了高达76倍和28倍。观察到普朗尼克F - 68的保护作用是浓度依赖性的：0.2%和0.3%（w/v）的F - 68分别使SF - 9细胞对剪切应力的抗性提高了15倍和42倍。然而，与0.3%（w/v）相比，将浓度增加到0.5%并没有显著提高细胞的抗性。0.2%的F - 68仅使TN - 368细胞的抗性提高了6倍。据信这种保护作用是聚合物吸附到细胞膜上的结果。所测试的聚合物添加剂均对SF - 9或TN - 368的生长速率没有显著影响。