Larionova N I, Kazanskaia N F, Sakharov I Iu, Berezin I V
Biokhimiia. 1979 Nov;44(11):2033-8.
Modification of pancreatic trypsin inhibitor by carboxymethyl dextran with the aid of carboxyl groups of the carrier activated by water-soluble carbodiimide has been carried out. A special role of electrostatic interactions of protein with the carrier during inhibitor modification has been shown. This modification results in active preparations with high protein concentration on the carrier; their molecular weight is practically equal to that of previously used carrier. A comparison of the thermal stability of high molecular weight preparations of pancreatic inhibitor with respect to protein concentration on the carrier and the number of links between protein and carrier has been carried out.
借助水溶性碳二亚胺活化载体的羧基，用羧甲基葡聚糖对胰蛋白酶抑制剂进行了修饰。研究表明了在抑制剂修饰过程中蛋白质与载体静电相互作用的特殊作用。这种修饰产生了载体上蛋白质浓度高的活性制剂；它们的分子量实际上与先前使用的载体相同。对载体上蛋白质浓度和蛋白质与载体之间连接数的胰抑制剂高分子量制剂的热稳定性进行了比较。
