Larionova N I, Kazanskaia N F, Sakharov I Iu, Mitiushina G V
Biokhimiia. 1980 Apr;45(4):683-6.
The activation of soluble polysaccharide matrices by titan tetrachloride was studied. A method for obtaining soluble preparations of pancreatic trypsin inhibitor bound to the activated polysaccharide matrix completely retaining its inhibiting activity was developed. It was shown that the equilibrium and kinetic constants for association of modified preparations of pancreatic inhibitor with commercial trypsin did not practically differ from those for native inhibitor. It was found that modification by titan tetrachloride-activated polysaccharides stabilized the protein molecule.
研究了四氯化钛对可溶性多糖基质的活化作用。开发了一种获得与活化多糖基质结合的胰腺胰蛋白酶抑制剂可溶性制剂的方法，该制剂完全保留其抑制活性。结果表明，修饰后的胰腺抑制剂制剂与市售胰蛋白酶结合的平衡常数和动力学常数与天然抑制剂的这些常数实际上没有差异。发现用四氯化钛活化的多糖进行修饰可稳定蛋白质分子。
