Maklakova I A, Valueva T A, Kolosova G V, Valuev L I, Mosolov V V
Prikl Biokhim Mikrobiol. 1983 Sep-Oct;19(5):654-8.
Covalent immobilization of the pancreatic trypsin inhibitor onto a polymeric carrier was accomplished by introducing a double C = C bond in the inhibitor with a subsequent copolymerization of the activized inhibitor with acrylamide and N,N'-methylenebisacrylamide. To prevent a loss of the antitryptic activity under acylation of lysine residues of the reactive centre, the inhibitor was preliminary bound to a complex with trypsin, which was destructed by acidifying the solution before copolymerization. The antitryptic activity of the immobilized inhibitor was shown to be equal to the activity of the inhibitor in the native state.
通过在胰蛋白酶抑制剂中引入双键C = C，随后将活化的抑制剂与丙烯酰胺和N，N'-亚甲基双丙烯酰胺进行共聚，将胰腺胰蛋白酶抑制剂共价固定在聚合物载体上。为防止反应中心赖氨酸残基酰化时抗胰蛋白酶活性丧失，抑制剂预先与胰蛋白酶形成复合物，在共聚前通过酸化溶液将其破坏。结果表明，固定化抑制剂的抗胰蛋白酶活性与天然状态下抑制剂的活性相当。
