de Leenheer A P, Cosyns-Duyck M C
J Chromatogr. 1979 Jul 1;174(2):325-39. doi: 10.1016/s0021-9673(00)86006-9.
Various procedures for converting 5-fluorouracil into its methyl, butyl and hexyl derivatives are described. Structures were established as the N,N'-dialkyl derivatives using mass spectrometry or combined gas-liquid chromatography-mass spectrometry. The reaction conditions, i.e., the amount of derivatization reagents and reaction time, were optimized. Gas-liquid chromatographic characteristics of the derivatives were investigated on different stationary liquid phases, and 2% or 3% SP-2250, 5% XE-60 and 5% OV-1 were found to be superior. With 5-chlorouracil as the internal standard a linear response for the various derivatives was observed in the microgram range. The applicability of the different dialkyl derivatives in the measurement of 5-fluorouracil in biological materials is discussed.
描述了将5-氟尿嘧啶转化为其甲基、丁基和己基衍生物的各种方法。通过质谱法或气相色谱-质谱联用确定结构为N,N'-二烷基衍生物。对反应条件，即衍生化试剂的用量和反应时间进行了优化。在不同的固定液上研究了衍生物的气相色谱特性，发现2%或3%的SP-2250、5%的XE-60和5%的OV-1效果更佳。以5-氯尿嘧啶为内标，在微克范围内观察到各种衍生物的线性响应。讨论了不同二烷基衍生物在生物材料中5-氟尿嘧啶测定中的适用性。
