2,6-二氨基甲苯和2,4-二氨基甲苯的高效液相色谱法及其在尿液和血浆中2,4-二氨基甲苯测定中的应用。

High-performance liquid chromatography of 2,6- and 2,4-diaminotoluene, and its application to the determination of 2,4-diaminotoluene in urine and plasma.

作者信息

Unger P D, Friedman M A

出版信息

J Chromatogr. 1979 Jul 1;174(2):379-84. doi: 10.1016/s0021-9673(00)86011-2.

Abstract

2,4-Diaminotoluene is used for the production of industrial dyes, and along with the 2,6-isomer, as an intermediate in the production of polyurethane foams. 2,6- and 2,4-diaminotoluene were resolved as sharp peaks by normal-phase high-performance liquid chromatography in 3 min by an acetonitrile-water-saturated chloroform elution solvent (8:2, v/v) with detection by ultraviolet absorbance at 250 nm. The relationship between peak height and amount injected was linear over a range of 0.025-2 microgram for both compounds. Retention times and peak heights were highly reproducible. Detection was very sensitive, allowing quantitation of 1-2 ng of either compound. Quantitative recovery of 2,4-diaminotoluene from spiked urine and plasma samples was obtained by extraction with methylene chloride.

摘要

2,4 - 二氨基甲苯用于生产工业染料，并且与2,6 - 异构体一起，作为生产聚氨酯泡沫的中间体。通过正相高效液相色谱法，使用乙腈 - 水 - 饱和氯仿洗脱溶剂（8:2，v/v），在3分钟内将2,6 - 和2,4 - 二氨基甲苯分离为尖锐峰，在250nm处通过紫外吸收进行检测。两种化合物在0.025 - 2微克范围内，峰高与进样量之间的关系呈线性。保留时间和峰高具有高度重现性。检测非常灵敏，可对两种化合物中的任何一种进行1 - 2纳克的定量分析。通过用二氯甲烷萃取，从加标尿液和血浆样品中获得了2,4 - 二氨基甲苯的定量回收率。