使用气相色谱-电子轰击正离子质谱法测定致癌物2,4-二氨基甲苯的血红蛋白加合物水平。
Determination of hemoglobin adduct levels of the carcinogen 2,4-diaminotoluene using gas chromatography-electron impact positive-ion mass spectrometry.
作者信息
Wilson P M, Hee S S, Froines J R
机构信息
UCLA Center for Occupational and Environmental Health, School of Public Health 90095-1772, USA.
出版信息
J Chromatogr B Biomed Appl. 1995 May 5;667(1):166-72. doi: 10.1016/0378-4347(94)00598-y.
A procedure to determine hemoglobin adduct yields resulting from exposure to the carcinogen 2,4-diaminotoluene (2,4-TDA) was developed using gas chromatography-electron impact positive-ion mass spectrometry. Liberated 2,4-TDA was quantified following alkaline hydrolysis of hemoglobin. Optimized derivatization of free 2,4-TDA with hepatafluorobutyric anhydride allowed detection of hemoglobin adduct levels as low as 5 ng/g Hb. Pure HFBA-2,4-TDA showed a linear dynamic range of 50 to 5000 pg. The quantitative extraction and recovery of liberated 2,4-TDA (ca. 100%) following hemoglobin hydrolysis allows accurate and precise determinations of adduct yields.
利用气相色谱-电子轰击正离子质谱法开发了一种测定接触致癌物2,4-二氨基甲苯（2,4-TDA）后血红蛋白加合物产量的方法。血红蛋白经碱性水解后，对释放出的2,4-TDA进行定量分析。用七氟丁酸酐对游离的2,4-TDA进行优化衍生化处理，可检测低至5 ng/g Hb的血红蛋白加合物水平。纯HFBA-2,4-TDA的线性动态范围为50至5000 pg。血红蛋白水解后释放出的2,4-TDA的定量提取和回收率（约100%）使得能够准确、精确地测定加合物产量。
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