Clerici E, Schechter I, Sela M
Immunology. 1970 Aug;19(2):267-75.
Antibodies of poly-D-alanyl specificity were produced in goats, rabbits, rats and mice by immunization with poly-D-alanyl ribonuclease. The reaction of the sera with formalinized sheep erythrocytes, tanned and coated with poly-D-alanyl human serum albumin, was inhibited with oligopeptides of D-alanine of increasing size. The upper limit of the size of goat and rabbit antibody combining sites was complementary to a tetrapeptide, in agreement with results obtained by inhibition of precipitin reaction. Since the data obtained with both methods compared favourably, the upper limit size of mouse and rat antibody combining sites of poly-D-alanyl specificity was estimated by the passive haemagglutination-inhibition technique. The results suggest that such sites are similar in size to those of rabbit and goat antibodies, and are capable of accommodating a peptide composed of four alanine residues.
通过用聚-D-丙氨酰核糖核酸酶免疫山羊、兔子、大鼠和小鼠，产生了具有聚-D-丙氨酰特异性的抗体。血清与用聚-D-丙氨酰人血清白蛋白鞣制和包被的甲醛化绵羊红细胞的反应，被大小不断增加的D-丙氨酸寡肽所抑制。山羊和兔子抗体结合位点大小的上限与一种四肽互补，这与通过抑制沉淀素反应获得的结果一致。由于用这两种方法获得的数据比较相符，因此通过被动血凝抑制技术估计了聚-D-丙氨酰特异性的小鼠和大鼠抗体结合位点的上限大小。结果表明，这些位点的大小与兔子和山羊抗体的位点相似，并且能够容纳由四个丙氨酸残基组成的肽。
