氧化磷酸化。三甲基锡和三乙基锡与大鼠肝脏线粒体的特异性结合。
Oxidative phosphorylation. The specific binding of trimethyltin and triethyltin to rat liver mitochondria.
作者信息
Aldridge W N, Street B W
出版信息
Biochem J. 1970 Jun;118(1):171-9. doi: 10.1042/bj1180171.
Abstract
- The binding of trimethyltin and triethyltin to rat liver mitochondria was determined and the results were analysed by the method of Scatchard (1949). 2. One binding site (site 1) has the correct characteristics for the site to which trimethyltin and triethyltin are attached when they inhibit oxidative phosphorylation. For each compound the concentration of site 1 is 0.8nmol/mg of protein and the ratios of their affinity constants are the same as the ratio of the concentrations inhibiting oxidative phosphorylation. 3. Binding site 1 is present in a fraction derived from mitochondria containing only 15% of the original protein. In this preparation ultrasonication rapidly destroyed site 1. 4. Dimethyltin and diethyltin do not prevent binding of triethyltin to rat liver mitochondria, whereas triethyl-lead does. 5. Trimethyltin and triethyltin bind to mitochondria from brown adipose tissue and the results indicate a binding site 1 similar to that in rat liver mitochondria. 6. The advantages and limitations of this approach to the study of inhibitors are discussed.
摘要
- 测定了三甲基锡和三乙基锡与大鼠肝脏线粒体的结合情况，并采用斯卡查德（1949年）的方法对结果进行了分析。2. 一个结合位点（位点1）具有三甲基锡和三乙基锡抑制氧化磷酸化时所附着位点的正确特征。对于每种化合物，位点1的浓度为0.8纳摩尔/毫克蛋白质，其亲和常数之比与抑制氧化磷酸化的浓度之比相同。3. 结合位点1存在于仅含原始蛋白质15%的线粒体衍生组分中。在此制剂中，超声处理会迅速破坏位点1。4. 二甲基锡和二乙基锡不会阻止三乙基锡与大鼠肝脏线粒体的结合，而三乙基铅会。5. 三甲基锡和三乙基锡与棕色脂肪组织的线粒体结合，结果表明存在一个与大鼠肝脏线粒体中类似的位点1。6. 讨论了这种研究抑制剂方法的优点和局限性。
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