Li Zhi-Hua, Li Ping, Shi Ze-Chao
Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Key Laboratory of Freshwater Biodiversity Conservation (Ministry of Agriculture), Key Field Station for Fishery Resource and Environment in Upper-Middle Reaches of Yangtze River (Ministry of Agriculture), Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
PLoS One. 2015 Apr 16;10(4):e0123091. doi: 10.1371/journal.pone.0123091. eCollection 2015.
The aim of the present study was to investigate the effect of Tributyltin (TBT) on brain function and neurotoxicity of freshwater teleost. The effects of long-term exposure to TBT on antioxidant related indices (MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GR, glutathione reductase; GPx, glutathione peroxidase), Na+-K+-ATPase and neurological parameters (AChE, acetylcholinesterase; MAO, monoamine oxidase; NO, nitric oxide) in the brain of common carp were evaluated. Fish were exposed to sublethal concentrations of TBT (75 ng/L, 0.75 μg/L and 7.5 μg/L) for 15, 30, and 60 days. Based on the results, a low level and short-term TBT-induced stress could not induce the notable responses of the fish brain, but long-term exposure (more than 15 days) to TBT could lead to obvious physiological-biochemical responses (based on the measured parameters). The results also strongly indicated that neurotoxicity of TBT to fish. Thus, the measured physiological responses in fish brain could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity.
本研究的目的是调查三丁基锡（TBT）对淡水硬骨鱼脑功能和神经毒性的影响。评估了长期暴露于TBT对鲤鱼脑中抗氧化相关指标（丙二醛，MDA；超氧化物歧化酶，SOD；过氧化氢酶，CAT；谷胱甘肽还原酶，GR；谷胱甘肽过氧化物酶，GPx）、钠钾ATP酶和神经学参数（乙酰胆碱酯酶，AChE；单胺氧化酶，MAO；一氧化氮，NO）的影响。将鱼暴露于亚致死浓度的TBT（75纳克/升、0.75微克/升和7.5微克/升）中15天、30天和60天。根据结果，低水平和短期的TBT诱导应激不会引起鱼脑的显著反应，但长期暴露（超过15天）于TBT会导致明显的生理生化反应（基于所测参数）。结果还强烈表明TBT对鱼具有神经毒性。因此，在鱼脑中测得的生理反应可为更好地理解TBT诱导的生物毒性机制提供有用信息。
