谷氨酰胺合成酶的放射化学测定法及该酶在大鼠组织中的活性。
A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues.
作者信息
Lund P
出版信息
Biochem J. 1970 Jun;118(1):35-9. doi: 10.1042/bj1180035.
Abstract
- A radiochemical assay for glutamine synthetase has been developed in which an ATP-regenerating system is incorporated to prevent accumulation of inhibitory amounts of ADP. It is particularly suitable for assay of the enzyme in crude tissue extracts containing high adenosine triphosphatase activity. 2. A survey of the distribution of the enzyme in tissues from normal male rats showed that activity is present in liver, brain cortex, kidney cortex, spleen, testis and retina. 3. The K(m) of the enzyme for l-glutamate is approx. 1.5x10(-2)m.
摘要
- 已开发出一种谷氨酰胺合成酶的放射化学测定法，其中引入了一个ATP再生系统以防止抑制量的ADP积累。它特别适用于测定含有高腺苷三磷酸酶活性的粗组织提取物中的该酶。2. 对正常雄性大鼠组织中该酶分布的调查表明，肝脏、大脑皮层、肾皮质、脾脏、睾丸和视网膜中存在活性。3. 该酶对L-谷氨酸的米氏常数约为1.5×10⁻²m。
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本文引用的文献
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Metabolism of amino-acids: The synthesis of glutamine from glutamic acid and ammonia, and the enzymic hydrolysis of glutamine in animal tissues.氨基酸的代谢：由谷氨酸和氨合成谷氨酰胺，以及动物组织中谷氨酰胺的酶促水解。
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Studies on the mechanism of glutamine synthesis; isolation and properties of the enzyme from sheep brain.谷氨酰胺合成机制的研究；绵羊脑组织中该酶的分离与特性
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