各种细菌中克氏锥虫因子和叶酸的存在情况。
Occurrence of Crithidia factors and folic acid in various bacteria.
作者信息
Iwai K, Kobashi M, Fujisawa H
出版信息
J Bacteriol. 1970 Oct;104(1):197-201. doi: 10.1128/jb.104.1.197-201.1970.
Abstract
Crithidia factors and folic acid were found to be widely distributed in culture fluids and in cells of 27 species of bacteria, when cultured under aerobic conditions into the stationary phase. Most bacteria excreted more Crithidia factors and folic acid than they retained in their cells. One Crithidia factor produced by Serratia indica and one produced by Bacillus cereus differed from biopterin in their chromatographic behavior. The factor excreted by S. indica appeared to be a 2-amino-4-hydroxy-6-substituted pteridine on the basis of KMnO(4) oxidation and ultraviolet absorption spectra. One of the folate compounds excreted by this organism was shown to be identical to 5,10-methylidynetetrahydrofolic acid by bioautography.
摘要
当在需氧条件下培养至稳定期时，发现克氏锥虫因子和叶酸广泛分布于27种细菌的培养液和细胞中。大多数细菌分泌的克氏锥虫因子和叶酸比它们细胞内保留的更多。印度沙雷氏菌产生的一种克氏锥虫因子和蜡样芽孢杆菌产生的一种克氏锥虫因子在色谱行为上与生物蝶呤不同。基于高锰酸钾氧化和紫外吸收光谱，印度沙雷氏菌分泌的因子似乎是一种2-氨基-4-羟基-6-取代蝶啶。通过生物自显影法表明，该生物体分泌的一种叶酸化合物与5,10-亚甲基四氢叶酸相同。
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