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脱氧核糖核酸消化产物的核苷酸“图谱”

Nucleotide 'maps' of digests of deoxyribonucleic acid.

作者信息

Murray K

出版信息

Biochem J. 1970 Aug;118(5):831-41. doi: 10.1042/bj1180831.

DOI:10.1042/bj1180831
PMID:5476726
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1179294/
Abstract

Various digests of (32)P-labelled DNA were examined by two-dimensional ionophoresis on cellulose acetate and DEAE-cellulose paper. The products from digestion with pancreatic deoxyribonuclease and Neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. Ionophoresis on DEAE-cellulose paper in triethylamine carbonate, pH 9.7, followed by ionophoresis in the second dimension at pH1.9 gave high resolution of nucleotides in very complex mixtures and permitted the fractionation of larger quantities than is possible on cellulose acetate. High resolution of nucleotides in compact spots was obtained with two-dimensional ionophoresis on cellulose acetate and AE-cellulose paper, a system that is a useful supplement to those based on DEAE-cellulose paper.

摘要

通过在醋酸纤维素和二乙氨基乙基纤维素纸上进行二维离子电泳，对（32）P标记的DNA的各种消化产物进行了检测。用胰脱氧核糖核酸酶和粗糙脉孢菌内切核酸酶消化得到的产物在性质上非常相似，但非常复杂，被用于研究各种离子电泳系统中核苷酸的图谱行为。在pH 9.7的碳酸三乙胺中于二乙氨基乙基纤维素纸上进行离子电泳，随后在pH 1.9下进行第二维离子电泳，可在非常复杂的混合物中实现核苷酸的高分辨率分离，并且能够分馏出比在醋酸纤维素上更多的量。在醋酸纤维素和AE纤维素纸上进行二维离子电泳可在紧密斑点中实现核苷酸的高分辨率，该系统是基于二乙氨基乙基纤维素纸的系统的有用补充。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/5890b0766950/biochemj00673-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/5f8251a8848c/biochemj00673-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b4b48598b2b9/biochemj00673-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/bfb0b50dec8a/biochemj00673-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b8fe79d6a624/biochemj00673-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/0654b4cf7b0a/biochemj00673-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b16a29b93d29/biochemj00673-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/5890b0766950/biochemj00673-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/5f8251a8848c/biochemj00673-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b4b48598b2b9/biochemj00673-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/bfb0b50dec8a/biochemj00673-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b8fe79d6a624/biochemj00673-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/0654b4cf7b0a/biochemj00673-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/b16a29b93d29/biochemj00673-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c0a/1179294/5890b0766950/biochemj00673-0171-a.jpg

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本文引用的文献

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Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.结晶脱氧核糖核酸酶；胸腺核酸的消化；反应动力学
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The deoxyribonucleases of Escherichia coli. I. Purification and properties of a phosphodiesterase.大肠杆菌的脱氧核糖核酸酶。I. 一种磷酸二酯酶的纯化及性质
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DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
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Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine.在浸渍有聚乙烯亚胺的纤维素薄层上对核酸消化产物进行色谱分析。
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Polynucleotides associated with histone preparations.与组蛋白制剂相关的多核苷酸。
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The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.噬菌体λDNA的3'末端核苷酸序列。
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Nucleotide sequencing of DNA: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage M13-ribosubstitution-DNA polymerase I-electrophoresis-two-dimensional fingerprinting).DNA的核苷酸测序：鸟嘌呤、胞嘧啶或腺嘌呤残基特异性切割产物的初步表征（噬菌体M13-核糖取代-DNA聚合酶I-电泳-二维指纹图谱）
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The behaviour of oligodeoxynucleotides on thin-layer chromatography on polyethyleneimine-cellulose and ion-exchange paper electrophoresis. Applications in fractionating and sequencing terminally labelled oligodeoxynucleotides.寡脱氧核苷酸在聚乙烯亚胺 - 纤维素薄层层析及离子交换纸电泳中的行为。在终末标记寡脱氧核苷酸的分级分离和测序中的应用。
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DNA nucleotide sequence restricted by the RI endonuclease.受RI核酸内切酶限制的DNA核苷酸序列。
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AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. II. STUDIES OF ENZYME SPECIFICITY.一种来自粗糙脉孢菌的对缺乏有序结构的多核苷酸具有特异性的核酸内切酶。II. 酶特异性研究。
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AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.一种来自粗糙脉孢菌的核酸内切酶，对缺乏有序结构的多核苷酸具有特异性。I. 酶的纯化及性质
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