Allen E G
Appl Microbiol. 1970 Aug;20(2):224-6. doi: 10.1128/am.20.2.224-226.1970.
Borrelia kansas and Plasmodium berghei have been stored after slow freezing in thioglycollate-glycerol medium for a 6-month period. During this time, 75% or more of the Borrelia remained motile, many intracellular malarial parasites exhibited amoeboid movement, and the growth pattern of both organisms in mice remained unchanged.
堪萨斯疏螺旋体和伯氏疟原虫在硫乙醇酸盐-甘油培养基中缓慢冷冻保存6个月。在此期间,75%或更多的疏螺旋体仍具运动性,许多细胞内疟原虫呈现阿米巴样运动,且两种生物体在小鼠体内的生长模式均未改变。