Effect of organ phospholipids on the growth of embryonal tissues cultured in vitro.

With regard to a set of studies about cellular nutrition, the effect of different amounts of phospholipids, extracted from various organs of calf (diencephalon, retina, cerebral cortex and heart), on normal embryonal chicken myocardium cultures has been tested. From the numerous observations we have made it is resulted that phospholipids, particularly at the highest levels, constantly decrease the culture migration velocity and this reduction is remarkably more accentuated in presence of 1.25%(0) heart phospholipids. Moreover in this condition the cells show a change in their morphology and appear all full of big lipid drops, perhaps in consequence of an alteration of the chemical-physical plasmalemma characteristics and a change in the membrane enzyme activities involving lipid metabolism too.