Cocchi M, Pignatti C, Olivo O M, Turchetto E
Biochem Exp Biol. 1980;16(2):107-9.
It has been valued the effects of different nutrients (glucose, phospholipids and ethanol) on the growth and morphology of chicken embryo cells, cultured in vitro by "pendent drop". Considering that the basal nourishing substratum used in these studies is the best medium that may be supplied to the cultures for the growth of this kind of tissues, the entity of damage induced by different nutrients has been shown. It may be said that the less negative effect is induced by 2.5% ethanol and by the association: "glucose + 2.5% ethanol", that the damage induced by only glucose seems to improve in presence of 2.5% ethanol, that in presence of phospholipids the cells appear all full of big lipid drops (especially in the condition: "phospholipid + ethanol") and that 5% ethanol deeply appears to impair and alter the cultures, as it was been observed in our previous experiences.
研究了不同营养物质（葡萄糖、磷脂和乙醇）对通过“悬滴法”体外培养的鸡胚细胞生长和形态的影响。鉴于这些研究中使用的基础营养培养基是可供此类组织生长的培养物的最佳培养基，已显示出不同营养物质诱导的损伤程度。可以说，2.5%乙醇以及“葡萄糖 + 2.5%乙醇”组合诱导的负面影响较小；在2.5%乙醇存在的情况下，仅葡萄糖诱导的损伤似乎有所改善；在存在磷脂的情况下，细胞似乎都充满了大脂滴（特别是在“磷脂 + 乙醇”的条件下）；并且5%乙醇似乎严重损害和改变了培养物，正如我们之前的实验所观察到的那样。
