Poal-Manresa J, Little K, Trueta J
Br J Exp Pathol. 1970 Aug;51(4):372-8.
Young guinea-pigs were given a scorbutic diet, and some subsequently allowed to recover. When killed, vessels were perfused with a mixture of Micropaque and Berlin blue. Walls of vessels formed during the period of ascorbic acid deficiency were weak. Sometimes bursts were surrounded by granulation tissue. Other weak patches burst as the perfusion medium was injected. All the sinusoid vessels were dilated, and blood flow in the bones was sluggish. Bone turnover was affected. The impaired blood flow caused resorption, in a manner analogous to bone removal in disuse osteoporosis. Impaired matrix production caused a cessation of osteoblastic activity. The resultant osteoporosis is particularly severe during growth. In the growth cartilage the calciflable matrix was defective, with its nucleation centres immediately available, so that some calcification was observed from the level of the base of the proliferative zone. Nucleoli in the osteogenic and connective tissue cells of scorbutic animals were abnormally large and dense. It is possible that they contained the RNA granules which would normally have been spread over endoplasmic reticulum membranes.
给幼年豚鼠喂食坏血病饮食，一些豚鼠随后被允许恢复。处死时，用泛影葡胺和柏林蓝的混合物灌注血管。在抗坏血酸缺乏期间形成的血管壁很脆弱。有时破裂处被肉芽组织包围。注入灌注介质时，其他薄弱部位也会破裂。所有的窦状血管都扩张了，骨骼中的血流缓慢。骨转换受到影响。血流受损导致吸收，其方式类似于废用性骨质疏松症中的骨质吸收。基质产生受损导致成骨细胞活性停止。由此产生的骨质疏松症在生长期间尤为严重。在生长软骨中，可钙化的基质有缺陷，其成核中心立即可见，因此从增殖区底部水平观察到一些钙化。患坏血病动物的成骨细胞和结缔组织细胞中的核仁异常大且致密。有可能它们含有通常会分布在内质网膜上的RNA颗粒。
