trans-4-Hydroxy-l-proline was found to occur in the bound state in the leaves of sandal (Santalum album L.), in which large amounts of free cis-4-hydroxy-l-proline are also present. 2. Bound trans-4-hydroxy-l-proline was present, associated mainly with a ;wall' fraction and a ;soluble' fraction roughly in equal proportions. 3. Bound proline is present only in small amounts in the ;soluble' fraction but is mostly associated with the ;wall' fraction and the other sedimented fractions. 4. In the free amino acid fraction more than 98% of the hydroxyproline had the cis-configuration, whereas in the ;wall' and ;soluble' fractions more than 90% of the bound hydroxyproline was in the trans-configuration. 5. Various extraction procedures indicated heterogeneity of the hydroxyproline-containing components. Hot 5% (w/v) trichloroacetic acid extracts about 25% of hydroxyproline and m-NaOH extracts an additional 25%. 6. Incorporation of [(14)C]proline into the bound hydroxyproline was demonstrated. The hydroxyproline component of the ;soluble' fraction does not appear to be the precursor of that of the ;wall' fraction.
摘要
反式-4-羟基-L-脯氨酸在檀香（Santalum album L.）叶片中以结合态存在，其中也含有大量游离的顺式-4-羟基-L-脯氨酸。2. 结合态反式-4-羟基-L-脯氨酸主要与“细胞壁”组分和“可溶性”组分相关，且比例大致相等。3. 结合态脯氨酸在“可溶性”组分中含量较少，主要与“细胞壁”组分及其他沉淀组分相关。4. 在游离氨基酸组分中，超过98%的羟脯氨酸具有顺式构型，而在“细胞壁”和“可溶性”组分中，超过90%的结合态羟脯氨酸为反式构型。5. 各种提取方法表明含羟脯氨酸的组分具有异质性。5%（w/v）热三氯乙酸可提取约25%的羟脯氨酸，间-氢氧化钠可再提取25%。6. 已证实[(14)C]脯氨酸掺入结合态羟脯氨酸中。“可溶性”组分中的羟脯氨酸组分似乎不是“细胞壁”组分中羟脯氨酸的前体。
