檀香（檀香属植物）中结合态反式-4-羟基-L-脯氨酸的研究。
Studies on bound trans-4-hydroxy-L-proline in sandal (Santalum album L.).
作者信息
Kuttan R, Radhakrishnan A N
出版信息
Biochem J. 1970 Oct;119(4):651-7. doi: 10.1042/bj1190651.
Abstract
- trans-4-Hydroxy-l-proline was found to occur in the bound state in the leaves of sandal (Santalum album L.), in which large amounts of free cis-4-hydroxy-l-proline are also present. 2. Bound trans-4-hydroxy-l-proline was present, associated mainly with a ;wall' fraction and a ;soluble' fraction roughly in equal proportions. 3. Bound proline is present only in small amounts in the ;soluble' fraction but is mostly associated with the ;wall' fraction and the other sedimented fractions. 4. In the free amino acid fraction more than 98% of the hydroxyproline had the cis-configuration, whereas in the ;wall' and ;soluble' fractions more than 90% of the bound hydroxyproline was in the trans-configuration. 5. Various extraction procedures indicated heterogeneity of the hydroxyproline-containing components. Hot 5% (w/v) trichloroacetic acid extracts about 25% of hydroxyproline and m-NaOH extracts an additional 25%. 6. Incorporation of [(14)C]proline into the bound hydroxyproline was demonstrated. The hydroxyproline component of the ;soluble' fraction does not appear to be the precursor of that of the ;wall' fraction.
摘要
- 反式-4-羟基-L-脯氨酸在檀香（Santalum album L.）叶片中以结合态存在，其中也含有大量游离的顺式-4-羟基-L-脯氨酸。2. 结合态反式-4-羟基-L-脯氨酸主要与“细胞壁”组分和“可溶性”组分相关，且比例大致相等。3. 结合态脯氨酸在“可溶性”组分中含量较少，主要与“细胞壁”组分及其他沉淀组分相关。4. 在游离氨基酸组分中，超过98%的羟脯氨酸具有顺式构型，而在“细胞壁”和“可溶性”组分中，超过90%的结合态羟脯氨酸为反式构型。5. 各种提取方法表明含羟脯氨酸的组分具有异质性。5%（w/v）热三氯乙酸可提取约25%的羟脯氨酸，间-氢氧化钠可再提取25%。6. 已证实[(14)C]脯氨酸掺入结合态羟脯氨酸中。“可溶性”组分中的羟脯氨酸组分似乎不是“细胞壁”组分中羟脯氨酸的前体。
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引用本文的文献
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Isolation and characterization of a hydroxyproline-containing protein from soluble extracts of the leaves of sandal (Santalum album L.).
Biochem J. 1974 Jul;141(1):147-53. doi: 10.1042/bj1410147.
本文引用的文献
1
Inhibition of formation of protein-bound hydroxyproline by free hydroxyproline in Avena coleoptiles.
Plant Physiol. 1967 Sep;42(9):1165-70. doi: 10.1104/pp.42.9.1165.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
The determination of collagen and elastin in tissues.
J Biol Chem. 1950 Oct;186(2):549-56.
4
A MICROMETHOD FOR THE DETERMINATION OF TRANSAMINASE ACTIVITY USING LIQUID SCINTILLATION COUNTING.
Clin Chim Acta. 1964 Sep;10:271-5. doi: 10.1016/0009-8981(64)90146-9.
5
Determination of D-amino acid oxidase activity in insect tissues using D-allohydroxyproline as substrate.
Biochim Biophys Acta. 1963 May 7;73:50-6. doi: 10.1016/0006-3002(63)90358-5.
6
Oxygen fixation into hydroxyproline of plant cell wall protein.
J Biol Chem. 1963 Apr;238:1438-40.
7
Biochemical chlorination in Streptomvces aureofaciens.
Nature. 1960 Nov 19;188:665. doi: 10.1038/188665a0.
8
Distribution and seasonal variation of allohydroxy-L-proline in Santalum album L.
Biochem J. 1961 Aug;80(2):378-84. doi: 10.1042/bj0800378.
9
A new method for isolation of hydroxy-L-proline and L-proline from gelatin.
J Biol Chem. 1959 Jul;234(7):1731-2.
10
14C-Proline and hydroxyproline in the protein metabolism of plants; an episode in the relation of metabolism to cell growth and morphogenesis.
Nature. 1958 Sep 27;182(4639):828-32. doi: 10.1038/182828a0.
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