Kasai M, Kometani T
Biochim Biophys Acta. 1979 Oct 19;557(1):243-7. doi: 10.1016/0005-2736(79)90106-8.
The permeabilities of sarcoplasmic reticulum vesicle membrane for various ions and neutral molecules were measured by following the change in light scattering intensity due to the osmotic volume change of the vesicles. 4-Acetoamido-4'-isothiocyanostilbene-2,2'-disulfonate (SITS), which is a potent inhibitor for the anion permeability of red blood cells membrane, inhibited the permeability of sarcoplasmic reticulum for anions such as Cl-, Pi and methanesulfonate, while it slightly increased that for cations and neutral molecules such as Na+, K+, choline and glycerol. Binding of 5 mumol SITS/g protein was necessary for the inhibition of anion permeability. These results suggest the existence of a similar anion transport system in sarcoplasmic reticulum membrane as revealed in red blood cell membrane.
通过跟踪由于囊泡渗透体积变化引起的光散射强度变化，测量了肌浆网囊泡膜对各种离子和中性分子的通透性。4-乙酰氨基-4'-异硫氰基芪-2,2'-二磺酸盐（SITS）是红细胞膜阴离子通透性的有效抑制剂，它抑制了肌浆网对Cl-、Pi和甲磺酸盐等阴离子的通透性，而对Na+、K+、胆碱和甘油等阳离子和中性分子的通透性略有增加。抑制阴离子通透性需要每克蛋白质结合5 μmol SITS。这些结果表明，肌浆网膜中存在与红细胞膜中类似的阴离子转运系统。
