White M M, Miller C
Biophys J. 1981 Aug;35(2):455-62. doi: 10.1016/S0006-3495(81)84801-1.
The Cl- permeability of membrane vesicles prepared from the electric organ of the marine ray Torpedo californica was studied by means of radioactive tracer exchange and by measuring the changes in the scattered-light intensity caused by osmotically induced volume changes. Both types of experiments indicate that a substantial fraction of the vesicles is extremely permeable of Cl-. Furthermore, this permeability pathway is inhibited by 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene, a well-known inhibitory of anion transport in a variety of systems. The properties of this permeability pathway are consistent with its identification as the voltage-aged Cl- channel studied in planar bilayers.
利用放射性示踪剂交换法以及通过测量由渗透压诱导的体积变化所引起的散射光强度变化，对从电鳐（Torpedo californica）电器官制备的膜泡的氯离子通透性进行了研究。这两类实验均表明，相当一部分膜泡对氯离子具有极高的通透性。此外，这种通透途径受到4,4'-二异硫氰基-2,2'-二磺酸芪的抑制，4,4'-二异硫氰基-2,2'-二磺酸芪是多种系统中著名的阴离子转运抑制剂。这种通透途径的特性与其被鉴定为在平面双层膜中研究的电压门控氯离子通道相一致。
