流感或新城疫病毒感染的单层细胞培养物中神经氨酸酶活性灶的定量测定
Quantitative determination of neuraminidase-active foci in cell monolayer cultures infected with influenza or newcastle disease virus.
作者信息
Palese P, Bodo G, Tuppy H
出版信息
J Virol. 1970 Oct;6(4):556-8. doi: 10.1128/JVI.6.4.556-558.1970.
Abstract
A synthetic neuraminidase substrate [2-(3'-methoxyphenyl)-N-acetyl-alpha-neuraminic acid] was used to detect myxo- and paramyxovirus replication in tissue culture. This method allowed estimation of viral growth even in the absence of cytopathogenic effects.
摘要
一种合成的神经氨酸酶底物[2-(3'-甲氧基苯基)-N-乙酰-α-神经氨酸]被用于检测组织培养中的黏液病毒和副黏液病毒复制。即使在没有细胞病变效应的情况下，这种方法也能对病毒生长进行评估。
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