Palese P, Bucher D, Kilbourne E D
Appl Microbiol. 1973 Feb;25(2):195-201. doi: 10.1128/am.25.2.195-201.1973.
A rapid and precise assay for neuraminidase using 2-(3'-methoxyphenyl)-N-acetyl-alpha-neuraminic acid (MPN) is described. It is proposed that this substrate be used for the standardization of activity of neuraminidases from viral, bacterial, and mammalian sources. MPN is also used as a chromogenic substrate to localize influenza and parainfluenza virus foci in tissue culture. This technique permits the recovery of infective virus from these stained "plaques." It has also been demonstrated that immunoprecipitin lines containing neuraminidase complexes with antibody in the Ouchterlony test can be observed by a similar staining procedure. No enzyme inhibition occurs in the presence of anti-neuraminidase antibodies or concanavalin A when MPN is used as a substrate in contrast to the results with high-molecular-weight substrates such as fetuin.
本文描述了一种使用2-(3'-甲氧基苯基)-N-乙酰-α-神经氨酸(MPN)对神经氨酸酶进行快速精确测定的方法。建议将该底物用于标准化来自病毒、细菌和哺乳动物来源的神经氨酸酶的活性。MPN还用作显色底物，以在组织培养中定位流感和副流感病毒病灶。该技术允许从这些染色的“噬菌斑”中回收感染性病毒。还证明了在Ouchterlony试验中，通过类似的染色程序可以观察到含有神经氨酸酶与抗体复合物的免疫沉淀线。与使用诸如胎球蛋白等高分子量底物的结果相反，当MPN用作底物时，在抗神经氨酸酶抗体或伴刀豆球蛋白A存在下不会发生酶抑制。
