Aranyi C, Fenters J, Tolkacz V
Appl Microbiol. 1970 Oct;20(4):633-7. doi: 10.1128/am.20.4.633-637.1970.
The scanning electron microscope (SEM) was used to detect changes in morphology of BSC-1 cells after infection with vesicular stomatitis virus (VSV) or herpes simplex virus. The morphological changes of the infected cells were related to the length of time of infection and to the virus used. Extensive alteration to the cytoplasm could be seen 24 and 48 hr after infection with 10 and 320 TCID(50) of VSV. Within 24 hr after infection with 1 TCID(50) of herpes simplex, a few nuclei were swollen. However, 72 hr after infection with 100 TCID(50) of herpesvirus, many nuclei were swollen and appeared in large aggregates, probably representing formation of a polykaryocyte. Corresponding samples stained with May-Grunwald-Giemsa were observed in the light microscope and morphological changes were compared to those seen with the SEM.
利用扫描电子显微镜（SEM）检测感染水疱性口炎病毒（VSV）或单纯疱疹病毒后BSC-1细胞的形态变化。被感染细胞的形态变化与感染时间长短以及所用病毒有关。用10和320个半数组织培养感染剂量（TCID50）的VSV感染后24小时和48小时，可观察到细胞质发生广泛改变。用1个TCID50的单纯疱疹病毒感染后24小时内，少数细胞核肿胀。然而，用100个TCID50的疱疹病毒感染72小时后，许多细胞核肿胀并聚集成大团块，可能代表多核细胞的形成。用May-Grunwald-Giemsa染色的相应样本在光学显微镜下观察，并将形态变化与SEM观察到的结果进行比较。
