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用直接荧光抗体法检测患白血病牛白细胞中的特异性抗原(作者译)

[Detection of specific antigen in the white blood cells of cattle with leukemia by a direct fluorescent antibody method (author's transl)].

作者信息

Ivanov V L

出版信息

Vopr Virusol. 1975 Sep-Oct(5):574-7.

PMID:54998
Abstract

The direct fluorescent antibody technique was used for detection of "specific" antigen in leukocytes of cattle with different forms of leukomia. Using fluorescein isothicoyanate-labeled immunoglobulins from colosterum of cows hyperimmunized with leukocyte mass from cattle with acute leukemia (hemocytobastosis), the "specific" antigen was detected in the cytoplasm of lymphoid and myeloid cells of animals with this and other forms of leukemia. This antigen was not detected in nuclear cells of the peripheral blood wither in normal animals or in animals with non-leukemic diseases (brucellosis, tuberculosis, various helminthic diseases) accompained by qualitative and quantitative changes in the blood similar to those leukemia.

摘要

采用直接荧光抗体技术检测患有不同形式白血病的牛白细胞中的“特异性”抗原。使用用患有急性白血病(成血细胞增多症)的牛的白细胞团超免疫的母牛初乳中异硫氰酸荧光素标记的免疫球蛋白,在患有这种及其他形式白血病的动物的淋巴和髓细胞的细胞质中检测到了“特异性”抗原。在正常动物或患有非白血病疾病(布鲁氏菌病、结核病、各种蠕虫病)且血液中伴有与白血病相似的质和量变化的动物的外周血核细胞中未检测到这种抗原。

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