甲状腺激素与磷脂膜的结合。
The binding of thyroid hormones to phospholipid membranes.
作者信息
Hillier A P
出版信息
J Physiol. 1970 Dec;211(3):585-97. doi: 10.1113/jphysiol.1970.sp009295.
Abstract
- Thyroxine and tri-iodothyronine are bound by liposomes (bimolecular phospholipid membranes produced by dispersing egg-yolk lecithin in water). At pH 7.4 the ;partition coefficient' between water and the phospholipid is 1.2 x 10(4) for thyroxine and 2.2 x 10(4) for tri-iodothyronine. The partition coefficient falls off rapidly in more alkaline solutions as the phenolic group on the hormones becomes ionized.2. The binding of thyroxine by membranes is very rapid, occurring at rates equivalent to those found in the binding of thyroxine by serum.3. Lecithin membranes are readily permeable to thyroxine (the oil:water partition coefficient is 1.7).4. The binding of thyroid hormones by perfused rat hearts and the binding of thyroid hormones by liposomes are similar in their sensitivity to pH. Simple alcoholic extracts of tissues have strong thyroxine-binding activity.5. These results are discussed in relation to the nature and function of the tissue thyroxine-binding reaction.
摘要
甲状腺素和三碘甲状腺原氨酸可与脂质体（通过将蛋黄卵磷脂分散于水中产生的双分子磷脂膜）结合。在pH 7.4时，甲状腺素在水与磷脂之间的“分配系数”为1.2×10⁴，三碘甲状腺原氨酸为2.2×10⁴。随着激素上的酚基团发生电离，在碱性更强的溶液中分配系数迅速下降。
甲状腺素与膜的结合非常迅速，其发生速率与甲状腺素与血清结合的速率相当。
卵磷脂膜对甲状腺素易于通透（油：水分配系数为1.7）。
灌注大鼠心脏对甲状腺激素的结合以及脂质体对甲状腺激素的结合，在对pH的敏感性方面相似。组织的简单醇提取物具有很强的甲状腺素结合活性。
结合组织甲状腺素结合反应的性质和功能对这些结果进行了讨论。
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