Stepanova L K, Sergeeva N S, Mel'nik E G, Andreeva V A, Demisova T P
Zh Mikrobiol Epidemiol Immunobiol. 1975 Oct(10):88-94.
The method of immunoelectrophoretic analysis was applied to the study of the antigenic structure of 180 freshly-isolated and laboratory cultures of salmonellae belonging to 29 serological types. A complicated set of specific and common antigens of bacteria was revealed in direct and cross experiments with the use of homologous and heterogenous antisera; immunophoregrams were drawn for each of the serological type under study. General regularities in immunophoretic, diffuse and serological characteristics of the individual antigens of salmonellae were established. It was shown that different serological types of salmonellae possessed surface K antigens along with the O and H antigens. On immunophoregrams K-antigens of salmonellae were revealed in the form of a bright precipitation arch in the central or the anode zone; O antigens gave a characteristic two-component precipitation line in the cathode zone of the phoregram.
免疫电泳分析方法被应用于对属于29种血清型的180株新鲜分离的沙门氏菌及实验室培养菌的抗原结构研究。在使用同源和异源抗血清进行的直接和交叉实验中，揭示了一组复杂的细菌特异性和共同抗原；为每种所研究的血清型绘制了免疫电泳图谱。确定了沙门氏菌各抗原在免疫电泳、扩散和血清学特征方面的一般规律。结果表明，不同血清型的沙门氏菌除了O和H抗原外还具有表面K抗原。在免疫电泳图谱上，沙门氏菌的K抗原以中央或阳极区明亮的沉淀弧形式显示；O抗原在电泳图谱的阴极区产生特征性的双组分沉淀线。
