Stepanova L K, Sergeeva N S
Zh Mikrobiol Epidemiol Immunobiol. 1977 Jun(6):62-7.
The authors studied 260 freshly isolated and laboratory cultures of salmonellae belonging to different serological groups. Heterogeneity of the majority of the strains studied by colour fluorescence of the colonies in the oblique light flux was demonstrated. Genetically bound spontaneous stable mutants with a different antigenic composition were selected by this method from a number of serological types. The isolated mutants were in the S-form and failed to differ by morphological, cultural and biochemical properties from the initial parental strains. A study of the antigenic structure by the method of agar immunoelectrophoresis and determination of their serological properties showed the mutants to have disturbances in the processes of O- or K-antigen synthesis.
作者研究了260株新分离的和实验室培养的属于不同血清学组的沙门氏菌。通过斜向光通量下菌落的颜色荧光证明了所研究的大多数菌株的异质性。通过这种方法从多种血清型中选择了具有不同抗原组成的遗传结合自发稳定突变体。分离出的突变体呈S型，在形态、培养和生化特性上与初始亲本菌株没有差异。通过琼脂免疫电泳法研究抗原结构并测定其血清学特性，结果表明突变体在O抗原或K抗原合成过程中存在干扰。
