[Do immunization of rabbits by N-acetylgalactosamine and a disaccharide linked to a protein produce anti-microbial antibodies (author's transl)].

Rabbits were immunized with N-acetylgalactosamine linked to bovalbumine. They produced antibodies which precipitated this same sugar linked to human gamma-globulins but did not agglutinate Salmonella johannesburg which carry a side chain of N-acetylgalactosamine. The same immunization enhanced the titre of "natural" antibodies agglutinating human A red cells (which carry a terminal N-acetylgalactosamine) but they did not evoke such antibodies in rabbits with no "natural" hemagglutinins. These negative results, when compared to the positive one obtained with 0-acetyl-3,6-dideoxygalactose suggest that rabbit antibody-sites limited to one sugar may exist but that they can be detected only under certain conditions. Another group of 8 rabbits was immunized with a disaccharide alpha-Glc-(1 leads to 6)-GalNAc linked to a protein. All produced antibodies agglutinating S. johannesburg (1,40) which carry this disaccharide and S. senftenberg which carry the disaccharide alpha-Glc-(1 leads to 6)-Gal. The titres of these antibodies decreased after a second course of immunization.