Benesch R, Edalji R, Benesch R E
J Biochem Biophys Methods. 1979 May;1(2):129-32. doi: 10.1016/0165-022x(79)90019-8.
A simple preparation of a deuterated alkylating reagent is described. Its reaction with the beta 93-SH groups of hemoglobin was used to illustrate the preparation of a protein derivative which contains 5 covalently attached non-exchangeable deuterium atoms at a unique locus on each beta chain.
本文描述了一种氘代烷基化试剂的简单制备方法。利用其与血红蛋白的β93-SH基团的反应来说明一种蛋白质衍生物的制备，该衍生物在每条β链的一个独特位点上含有5个共价连接的不可交换氘原子。
