Venkatachalam M A, Cotran R S, Karnovsky M J
J Exp Med. 1970 Dec 1;132(6):1168-80. doi: 10.1084/jem.132.6.1168.
Beef liver catalase (mol wt 240,000) was injected intravenously into normal rats and rats made nephrotic with aminonucleoside of puromycin. The localization of the tracer in the kidneys was then studied by ultrastructural cytochemistry, 3 min-12 hr after injection. Passage of catalase into the urinary space in normal rats was restricted by the basement membrane and by the epithelial slit pore. Nephrotic glomeruli showed extensive fusion of foot processes and formation of pockets and vacuoles in the fused epithelium; within 3 min after injection, catalase appeared in basal pockets, epithelial vacuoles, and the urinary space. Residual slit pores and close junctions in fused epithelium were impermeable to catalase. These studies indicate that alteration of the epithelial cells and basement membrane is responsible for protein leakage in aminonucleoside nephrosis.
将牛肝过氧化氢酶（分子量240,000）静脉注射到正常大鼠和用嘌呤霉素氨基核苷诱导产生肾病的大鼠体内。然后在注射后3分钟至12小时，通过超微结构细胞化学研究示踪剂在肾脏中的定位。在正常大鼠中，过氧化氢酶进入尿腔受到基底膜和上皮裂孔的限制。肾病性肾小球显示足突广泛融合，融合上皮中形成囊袋和空泡；注射后3分钟内，过氧化氢酶出现在基底囊袋、上皮空泡和尿腔中。融合上皮中残留的裂孔和紧密连接对过氧化氢酶是不可渗透的。这些研究表明，上皮细胞和基底膜的改变是氨基核苷肾病中蛋白质渗漏的原因。
