Kanwar Y S, Jakubowski M L
Kidney Int. 1984 Apr;25(4):613-8. doi: 10.1038/ki.1984.65.
Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of 125I-cationic ferritin (CFI, pI approximately 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 +/- 0.04, 1.49 +/- 0.05, 1.50 +/- 0.05, and 1.51 +/- 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.
采用定量电子显微镜放射自显影术来确定肾病诱发后大鼠肾小球基底膜（GBM）阴离子位点与125I-阳离子铁蛋白（CFI，pI约为7.2至7.4）结合的变化。通过单次静脉注射嘌呤霉素氨基核苷（PAN）使动物患肾病，并在给药后0、7、14和21天处死。静脉注射CFI（10 mg/mCi/100 g体重）。随后通过灌注固定肾脏并进行电子显微镜放射自显影处理。PAN肾病0、7、14和21天时GBM上的平均颗粒密度分别为1.50±0.04、1.49±0.05、1.50±0.05和1.51±0.04。这些结果表明，在PAN肾病的整个过程中，富含硫酸乙酰肝素蛋白聚糖的阴离子位点没有显著改变。
