大鼠肝脏过氧化物酶体的合成与周转。3. 过氧化物酶体的大小分布及新过氧化氢酶的掺入。
The synthesis and turnover of rat liver peroxisomes. 3. The size distribution of peroxisomes and the incorporation of new catalase.
作者信息
Poole B, Higashi T, De Duve C
出版信息
J Cell Biol. 1970 May;45(2):408-15. doi: 10.1083/jcb.45.2.408.
Abstract
Rat liver peroxisomes have been separated according to size by zonal sedimentation. A method is described for calculating the size of the particles from their final position in the gradient. Peroxisomes seem biochemically homogeneous throughout their size distribution. 3 hr after injection of tritiated leucine, the specific radioactivity of catalase is the same in peroxisomes of different sizes, and it remains so for up to 1 wk after administration of the precursor. This observation rules out the possibility that peroxisomes have an extended period of independent growth. If individual particles maintain an independent existence, they must be formed very rapidly. The other possible explanation is that peroxisomes exchange material within the liver cell.
摘要
大鼠肝脏过氧化物酶体已通过区带沉降法按大小分离。本文描述了一种根据颗粒在梯度中的最终位置计算其大小的方法。过氧化物酶体在其整个大小分布范围内在生化性质上似乎是均匀的。注射氚标记的亮氨酸3小时后，不同大小的过氧化物酶体中过氧化氢酶的比放射性相同，并且在前体给药后长达1周内一直保持如此。这一观察结果排除了过氧化物酶体有较长独立生长周期的可能性。如果单个颗粒保持独立存在，它们必定是非常快速地形成的。另一种可能的解释是过氧化物酶体在肝细胞内进行物质交换。
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The synthesis and turnover of rat liver peroxisomes. 3. The size distribution of peroxisomes and the incorporation of new catalase.大鼠肝脏过氧化物酶体的合成与周转。3. 过氧化物酶体的大小分布及新过氧化氢酶的掺入。
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本文引用的文献
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The kinetics of catalase synthesis and destruction in vivo.过氧化氢酶在体内合成与分解的动力学。
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Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
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Peroxisomes (microbodies and related particles).过氧化物酶体（微体及相关颗粒）。
Physiol Rev. 1966 Apr;46(2):323-57. doi: 10.1152/physrev.1966.46.2.323.
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Biogenesis and turnover of rat liver peroxisomes.大鼠肝脏过氧化物酶体的生物发生与周转
Ann N Y Acad Sci. 1969 Dec 19;168(2):229-43. doi: 10.1111/j.1749-6632.1969.tb43112.x.
8
The synthesis and turnover of rat liver peroxisomes. I. Fractionation of peroxisome proteins.大鼠肝脏过氧化物酶体的合成与周转。I. 过氧化物酶体蛋白的分级分离。
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