Theodoridis A, Nevill E M, Els H J, Boshoff S T
Onderstepoort J Vet Res. 1979 Dec;46(4):191-8.
Five viruses, unrelated to bovine ephemeral fever virus (BEFV), were isolated from Culicoides biting-midges collected during the summer months of the years 1968-69 and 1969-70 near a cattle herd in which cases of BEF occurred and at an open horse stable at Onderstepoort. These viruses were investigated by means of serological, electron-microscopical and physicochemical tests. It was established that 2 isolates, Cul. 1/69 and Cul. 2/69, were related to each other and belonged to the Palyam subgroup of the genus Orbivirus, that isolate Cul. 3/69 belonged to the equine encephalosis subgroup of the genus Orbivirus, while Cul. 1/70 was related to Akabane virus, which belongs to the Simbu subgroup of the family Bunyaviridae. One isolate, Cul. 5/69, though prevalent in the cattle population, could not be identified at this point. A brief serological survey indicated that the cattle in the nearby herd possessed antibodies against all the isolates except Cul. 3/69. BEFV could not be isolated in mice or in cultured cells from the wild-caught Culicoides.
1968 - 1969年和1969 - 1970年夏季，在发生牛流行热病例的牛群附近以及翁德斯托普特的一个开放式马厩采集的库蠓叮咬蠓中，分离出了5种与牛流行热病毒（BEFV）无关的病毒。通过血清学、电子显微镜和物理化学测试对这些病毒进行了研究。结果确定，2个分离株Cul. 1/69和Cul. 2/69彼此相关，属于环状病毒属的帕利亚姆亚组；分离株Cul. 3/69属于环状病毒属的马脑脊髓炎亚组；而Cul. 1/70与赤羽病毒相关，赤羽病毒属于布尼亚病毒科的辛布亚组。一个分离株Cul. 5/69虽然在牛群中普遍存在，但此时无法鉴定。一项简短的血清学调查表明，附近牛群中的牛对除Cul. 3/69之外的所有分离株都具有抗体。在小鼠或培养细胞中，无法从野外捕获的库蠓中分离出BEFV。
