Radtke H W, Rege A B, LaMarche M, Fisher J W
Proc Clin Dial Transplant Forum. 1979;9:179-83.
Using the fetal mouse liver cell culture technique for evaluation of erythroid colony formation (CFU-E) sera of 15 patients with CRF and 10 normal subjects were investigated. When sera were removed immediately prior to the onset of regular hemodialysis from patients in a severe uremic state, they produced a significant inhibition of CFU-E formation when tested in the fetal mouse liver cell culture. In contrast, when the same sera after 48 hrs of in vitro dialysis were tested, inhibition of CFU-E formation was reversed to a large part. Inhibition was reversed in the same manner when sera removed from the same patients after 16--20 wks of regular hemodialysis therapy were tested in the fetal mouse liver cell culture. Thus, the EIF present in the serum of patients were severe uremia can be removed by in vivo and in vitro dialysis.
采用胎鼠肝细胞培养技术评估15例慢性肾功能衰竭患者和10名正常受试者的红系集落形成（CFU-E）血清。当在严重尿毒症状态的患者开始定期血液透析之前立即采集血清时，在胎鼠肝细胞培养中检测发现，这些血清对CFU-E的形成有显著抑制作用。相反，当检测同一批血清在体外透析48小时后的情况时，CFU-E形成的抑制作用在很大程度上得到了逆转。当检测从接受16 - 20周定期血液透析治疗后的同一批患者采集的血清时，抑制作用以同样的方式得到了逆转。因此，严重尿毒症患者血清中存在的促红细胞生成抑制因子可通过体内和体外透析去除。
