抗生物素蛋白的纯化与结晶
Purification and crystallization of avidin.
作者信息
Green N M, Toms E J
出版信息
Biochem J. 1970 Jun;118(1):67-70. doi: 10.1042/bj1180067.
Abstract
An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1mug of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx. 66000) contained four identical subunits.
摘要
本文描述了一种从鸡蛋白中提纯抗生物素蛋白的改进方法，包括结晶法。该产品每毫克可结合15.1微克生物素，相当于16200的当量重量。氨基酸组成显示每16200克有整数个氨基酸残基，这表明抗生物素蛋白的每个分子（分子量约为66000）包含四个相同的亚基。
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Biochem J. 1970 Jun;118(1):71-2. doi: 10.1042/bj1180071.
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