Foody L T, Niehoff M L, D'Agrosa L S, Connors N A, Sullivan J M
J Auton Nerv Syst. 1979 Oct;1(1):77-91. doi: 10.1016/0165-1838(79)90007-9.
Previous functional and anatomical techniques have characterized the cardiac nerves and plexuses. They cannot, however, determine the course of fibers arising from a specific ganglion. This study has found that the intraaxonal orthograde labeling of axons can be used to determine the course of postganglionic sympathetic cardiac fibers in the dog and cat. The canine left caudal cervical ganglion and the feline right stellate ganglion were exposed through appropriate thoracotomies. Each ganglion received multiple injections of tritiated leucine (500 muCi/animal). Following a 3--14-day survival period the anesthetized animals were sacrificed by vascular perfusion. The injected ganglia, extracardiac nerves and selected portions of the heart were processed for autoradiography. Autoradiographs from the dog demonstrated labeled postganglionic sympathetic nerves in the extracardiac plexus, left atrial epicardium and the epicardium beneath the coronary sulcus. Labeled nerves in the cat heart were found within the epicardial layers and associated with blood vessels in the left ventricular myocardium. Neither myelinated fibers traveling through the injection site nor intrinsic cardiac ganglion cells were labeled, although the latter were often closely approximated to heavily labeled fibers.
以往的功能和解剖学技术已对心脏神经和神经丛进行了特征描述。然而，它们无法确定源自特定神经节的纤维走向。本研究发现，轴突内顺行标记可用于确定犬和猫节后交感心脏纤维的走向。通过适当的开胸手术暴露犬的左颈尾神经节和猫的右星状神经节。每个神经节接受多次氚标记亮氨酸注射（500微居里/动物）。在3至14天的存活期后，通过血管灌注处死麻醉的动物。对注射的神经节、心外神经和心脏的选定部分进行放射自显影处理。犬的放射自显影片显示心外神经丛、左心房心外膜和冠状沟下方的心外膜中有标记的节后交感神经。在猫心脏的心外膜层内以及与左心室心肌中的血管相关处发现了有标记的神经。尽管后者常常与标记密集的纤维紧密相邻，但穿过注射部位的有髓纤维和心脏固有神经节细胞均未被标记。
