Kupferberg H J, Longacre-Shaw J
Ther Drug Monit. 1979;1(1):117-22. doi: 10.1097/00007691-197901000-00012.
Plasma mephobarbital and phenobarbital concentrations were determined in 11 epileptic patients receiving mephobarbital alone or in combination with other antiepileptic drugs. The analysis was carried out by a selective ion monitoring (SIM) mass fragmentography technique following formation of N-propyl derivatives of both drugs. The plasma concentrations of phenobarbital ranged from 4 to 32 micrograms/ml and those of mephobarbital from 0.2 to 1.7 micrograms/ml. Differences in the metabolism rates of the drugs accounted for the plasma concentration differences; mephobarbital is metabolized more rapidly than phenobarbital. Phenobarbital concentrations obtained by SIM mass fragmentography were similar to those obtained by gas-liquid chromatographic on-column methylation, which quantitates only "total phenobarbital" (mephobarbital plus phenobarbital).
对11例单独服用美索比妥或与其他抗癫痫药物联合使用美索比妥的癫痫患者测定了血浆中美索比妥和苯巴比妥的浓度。在两种药物形成N-丙基衍生物后，采用选择性离子监测（SIM）质谱碎片分析法进行分析。苯巴比妥的血浆浓度范围为4至32微克/毫升，美索比妥的血浆浓度范围为0.2至1.7微克/毫升。药物代谢率的差异导致了血浆浓度的差异；美索比妥的代谢比苯巴比妥更快。通过SIM质谱碎片分析法获得的苯巴比妥浓度与通过气液色谱柱上甲基化获得的浓度相似，后者仅对“总苯巴比妥”（美索比妥加苯巴比妥）进行定量。
