Carbini L, Lantini T, Orgiana G, Scarpa A L, Padalino A
Boll Soc Ital Biol Sper. 1979 Jul 15;55(12):1196-202.
Albino rats (Sprague-Dawley) of mean weight 100 g were divided into four groups and given for 7 days a balanced diet. They were then placed in metabolic cages for fifteen days and fed diets containing different quantities of casein: 18% (D18), 36% (D36), 50% (D50) and 72% (D72). The levels of total calcium, inorganic phosphorus, alkaline phosphatase activity, total proteins and urea were determined. The urinary and fecal excretion of calcium were determined on specimens of urine and stool collected every two days. The metabolic balance of nitrogen was also estimated. The results show there is not a linear relationship between a high protein diet and plasma protein levels, but a progressive body calcium loss was observed with the increase of casein in the diet, which confirms what other workers have already suggested.
平均体重为100克的白化大鼠（斯普拉格-道利大鼠）被分成四组，给予7天的均衡饮食。然后将它们置于代谢笼中15天，并喂食含有不同量酪蛋白的饮食：18%（D18）、36%（D36）、50%（D50）和72%（D72）。测定了总钙、无机磷、碱性磷酸酶活性、总蛋白和尿素的水平。每两天收集尿液和粪便样本，测定钙的尿排泄量和粪排泄量。还估算了氮的代谢平衡。结果表明，高蛋白饮食与血浆蛋白水平之间不存在线性关系，但随着饮食中酪蛋白含量的增加，观察到机体钙逐渐流失，这证实了其他研究人员已经提出的观点。
