Borchardt K A, Richardson J A
Br Med J. 1971 Jan 23;1(5742):205-6. doi: 10.1136/bmj.1.5742.205.
The influence of human plasma on the antibacterial effect of solutions for peritoneal dialysis was studied. The solutions contained 43 mEq per litre of either acetate or lactate as the source of base. Enough pooled human plasma was added to half of each solution to give a total concentration of a gramme of protein per litre. The numbers of viable organisms from 15 clinical isolates each of Staphylococcus aureus, Escherichia coli, and Pseudomonas species were counted before and after incubation in the four solutions. Numbers of viable Staph. aureus and E. coli diminished consistently after incubation in all four solutions, but the greatest decreases occurred in the acetate solution which contained no plasma. Plasma abolished the greater antibacterial effect of acetate on these organisms. Differences between numbers of viable Pseudomonas sp. after incubation in the four solutions were not significant. The diffusion of substances from plasma into dialysis fluids during peritoneal dialysis, therefore, may abolish the greater antibacterial effect of solutions made with acetate rather than lactate.
研究了人血浆对腹膜透析液抗菌效果的影响。这些透析液每升含有43毫当量的醋酸盐或乳酸盐作为碱源。向每种透析液的一半中加入足够的混合人血浆，使蛋白质总浓度达到每升1克。在四种透析液中孵育前后，对15株金黄色葡萄球菌、大肠杆菌和假单胞菌属临床分离株的活菌数进行计数。在所有四种透析液中孵育后，金黄色葡萄球菌和大肠杆菌的活菌数持续减少，但在不含血浆的醋酸盐溶液中减少最多。血浆消除了醋酸盐对这些微生物更强的抗菌作用。四种透析液中孵育后假单胞菌属活菌数的差异不显著。因此，在腹膜透析过程中，物质从血浆扩散到透析液中，可能会消除用醋酸盐而非乳酸盐配制的透析液更强的抗菌作用。
