Moscona A A
Science. 1971 Mar 5;171(3974):905-7. doi: 10.1126/science.171.3974.905.
Embryonic tissue cells dissociated with ethylenediaminetetraacetate are readily agglutinated by the carbohydrate-binding protein concanavalin A. In this property, they resemble transformed, neoplastic cells; and they differ from untransformed adult cells, which are agglutinated by concanavalin A only after their receptors are unmasked by proteolytic treatment. Receptor sites for wheat germ agglutinin are also present on the surface of embryonic cells, but in a masked form, as on untransformed adult culture cells; they can be unmasked by treatment of the cells with trypsin. Concanavalin A binding sites on embryonic cells may function in cell contact and cell organization during embryonic morphogenesis and differentiation and later become masked in adult cells. The unmasking of these sites in neoplastic cells may represent a return, in this respect, to a condition resembling that of embryonic cells and may be related to cell mobility associated with infiltration and metastasis.
用乙二胺四乙酸解离的胚胎组织细胞很容易被碳水化合物结合蛋白伴刀豆球蛋白A凝集。在这一特性上，它们类似于转化的肿瘤细胞；并且它们不同于未转化的成年细胞，未转化的成年细胞只有在其受体被蛋白水解处理暴露后才会被伴刀豆球蛋白A凝集。小麦胚凝集素的受体位点也存在于胚胎细胞表面，但呈隐蔽形式，就像未转化的成年培养细胞一样；它们可以通过用胰蛋白酶处理细胞而被暴露。胚胎细胞上的伴刀豆球蛋白A结合位点可能在胚胎形态发生和分化过程中的细胞接触和细胞组织中起作用，随后在成年细胞中被掩盖。在肿瘤细胞中这些位点的暴露在这方面可能代表着回归到类似于胚胎细胞的状态，并且可能与浸润和转移相关的细胞迁移有关。
