温度敏感型猿猴病毒40转化细胞的表面变化
Surface changes in temperature-sensitive Simian virus 40-transformed cells.
作者信息
Noonan K D, Renger H C, Basilico C, Burger M M
出版信息
Proc Natl Acad Sci U S A. 1973 Feb;70(2):347-9. doi: 10.1073/pnas.70.2.347.
Abstract
A binding assay that shows consistent differences in the amounts of tritium-labeled concanavalin A that bind to normal and virally transformed cells was used to study the kinetic changes of cell surface in SV40-transformed 3T3 cells that express the transformed phenotype in a temperature-sensitive manner (tsSV3T3 cells). The increase in concanavalin A binding, which paralleled the appearance of the characteristics of transformed cells, was dependent on the synthesis of cellular DNA. In agreement with the results of binding studies, exponentially growing tsSV3T3 cells agglutinated at much lower lectin titers (concanavalin A as well as wheat-germ agglutinin) at 32 degrees than at 39 degrees .
摘要
一种结合试验显示，与正常细胞和病毒转化细胞结合的氚标记伴刀豆球蛋白A的量存在一致差异，该试验用于研究温度敏感型表达转化表型的SV40转化3T3细胞（tsSV3T3细胞）中细胞表面的动力学变化。伴刀豆球蛋白A结合的增加与转化细胞特征的出现平行，这依赖于细胞DNA的合成。与结合研究结果一致，指数生长的tsSV3T3细胞在32℃时比在39℃时以低得多的凝集素效价（伴刀豆球蛋白A以及麦胚凝集素）发生凝集。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1971 Feb;68(2):283-6. doi: 10.1073/pnas.68.2.283.
2
Retransformation of ts SV40 transformants by murine sarcoma virus at non-permissive temperature.
Nat New Biol. 1972 Nov 1;240(96):19-21. doi: 10.1038/newbio240019a0.
3
Quantitative binding of 125 I-concanavalin A to normal and transformed cells.125I-伴刀豆球蛋白A与正常细胞和转化细胞的定量结合。
J Virol. 1971 Nov;8(5):716-21. doi: 10.1128/JVI.8.5.716-721.1971.
4
A common biochemical change in SV40 and polyoma virus transformed mouse cells coupled to control of cell growth in culture.SV40和多瘤病毒转化的小鼠细胞中与培养物中细胞生长控制相关的常见生化变化。
Virology. 1971 Oct;46(1):60-72. doi: 10.1016/0042-6822(71)90006-7.
5
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7
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8
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