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一种鉴别测定血浆抗凝血酶的方法。

A method for the differential determination of plasma antithrombins.

作者信息

Musumeci V, Vincenti A, Bizzi B

出版信息

J Clin Pathol. 1976 Jan;29(1):63-8. doi: 10.1136/jcp.29.1.63.

DOI:10.1136/jcp.29.1.63
PMID:55420
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC475948/
Abstract

A method for the differential determination of plasma antithrombins, antithrombin III and alpha2 macroglobulin, is described. The method is based on the selective inactivation of plasma alpha2 macroglobulin by treatment with 0-1 M methylamine for 10 minutes at 37 degrees C and on the observation that antithrombin III and alpha2 macroglobulin inhibited in defibrinated plasma low concentrations of thrombin without mutual interference and according to pseudo-first order reaction. In healthy subjects antithrombin III was shown to account for about 70% of the total antithrombin activity. But in patients with liver cirrhosis, where low levels of total antithrombin activity were observed, the relative contribution of antithrombin III was found to be noticeably lower.

摘要

本文描述了一种鉴别测定血浆抗凝血酶、抗凝血酶III和α2巨球蛋白的方法。该方法基于以下原理：通过在37℃下用0 - 1M甲胺处理10分钟来选择性灭活血浆α2巨球蛋白，并且观察到抗凝血酶III和α2巨球蛋白在去纤维蛋白血浆中能抑制低浓度凝血酶，且互不干扰，遵循假一级反应。在健康受试者中，抗凝血酶III约占总抗凝血酶活性的70%。但在观察到总抗凝血酶活性水平较低的肝硬化患者中，发现抗凝血酶III的相对贡献明显较低。

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A method for the differential determination of plasma antithrombins.一种鉴别测定血浆抗凝血酶的方法。
J Clin Pathol. 1976 Jan;29(1):63-8. doi: 10.1136/jcp.29.1.63.
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Novel immunoenzyme assays for thrombin-antithrombin III and thrombin-alpha 2-macroglobulin complexes in plasma.血浆中凝血酶-抗凝血酶III及凝血酶-α2-巨球蛋白复合物的新型免疫酶测定法。
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本文引用的文献

1
Studies on the antithrombin and heparin cofactor activities of a fraction adsorbed from plasma by aluminum hydroxide.关于用氢氧化铝从血浆中吸附的一个组分的抗凝血酶和肝素辅因子活性的研究。
Circ Res. 1955 Jul;3(4):397-402. doi: 10.1161/01.res.3.4.397.
2
Antithrombin 3, antifactor Xa and heparin.抗凝血酶3、抗Xa因子与肝素。
Br J Haematol. 1970 Sep;19(3):283-305. doi: 10.1111/j.1365-2141.1970.tb01627.x.
3
Binding of thrombin to antithrombin III.凝血酶与抗凝血酶III的结合。
Scand J Clin Lab Invest. 1969 Aug;24(1):23-7. doi: 10.3109/00365516909080127.
4
[Antiproteinase activity of alpha-2-macroglobulin. II. Its role in progressive antithrombin states].[α-2-巨球蛋白的抗蛋白酶活性。II. 其在进行性抗凝血酶状态中的作用]
Rev Fr Etud Clin Biol. 1968 Feb;13(2):179-86.
5
Immunological studies on human antithrombin 3. Influence of age, sex and use of oral contraceptives on serum concentration.人抗凝血酶3的免疫学研究。年龄、性别及口服避孕药的使用对血清浓度的影响。
Scand J Haematol. 1970;7(1):10-7.
6
The purification and mechanism of action of human antithrombin-heparin cofactor.人抗凝血酶-肝素辅因子的纯化及其作用机制
J Biol Chem. 1973 Sep 25;248(18):6490-505.
7
Deficiency of antithrombin 3 activity associated with hereditary thrombosis tendency.抗凝血酶3活性缺乏与遗传性血栓形成倾向相关。
J Med. 1972;3(6):349-58.
8
The plasmin inhibitor of human plasma. IV. Its action on plasmin, trypsin, chymotrypsin, and thrombin.人血浆中的纤溶酶抑制剂。IV. 其对纤溶酶、胰蛋白酶、糜蛋白酶和凝血酶的作用。
J Biol Chem. 1966 Nov 10;241(21):5102-7.
9
Alpha-1-antitrypsin, an inhibitor for thrombin and plasmin.α-1-抗胰蛋白酶，一种凝血酶和纤溶酶的抑制剂。
Clin Chim Acta. 1967 Jul;17(1):111-7. doi: 10.1016/0009-8981(67)90104-0.
10
Dissociation of esterolytic and clotting activities of thrombin by trypsin-binding macroglobulin.胰蛋白酶结合巨球蛋白对凝血酶酯解活性和凝血活性的解离作用
Proc Soc Exp Biol Med. 1966 Feb;121(2):444-9. doi: 10.3181/00379727-121-30800.

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