Bottino N R
J Lipid Res. 1971 Jan;12(1):24-30.
The fractionation of marine-oil triglycerides according to their degree of unsaturation was achieved on silica gel thin layers with 8% silver nitrate (w/w). Under the optimum conditions, cod-liver and whale oils were reproducibly separated into seven and six fractions, respectively. The fatty acid compositions of the fractions obtained from cod-liver and whale oils were further studied by gas-liquid chromatography. The following was found in both oils: Saturated and monoenoic acids were not only abundant in their corresponding fractions, but also comprised about two-thirds of the fatty acids in the more unsaturated fractions. Instead, polyenoic fatty acids of similar degrees of unsaturation predominated only in the particular fraction which corresponded to their number of double bonds. Thus, the distribution of fatty acids of varying degrees of unsaturation among marine triglycerides is not random.
在含有8%硝酸银（重量/重量）的硅胶薄层上，根据海洋油甘油三酯的不饱和度对其进行了分馏。在最佳条件下，鳕鱼肝油和鲸油分别可重复地分离成七个和六个馏分。通过气液色谱法进一步研究了从鳕鱼肝油和鲸油中获得的馏分的脂肪酸组成。在这两种油中都发现了以下情况：饱和脂肪酸和单烯酸不仅在其相应的馏分中含量丰富，而且在更不饱和的馏分中还占脂肪酸的约三分之二。相反，具有相似不饱和度的多烯脂肪酸仅在与其双键数量相对应的特定馏分中占主导地位。因此，不同不饱和度的脂肪酸在海洋甘油三酯中的分布不是随机的。
