产蛋母鸡肝脏、蛋黄和脂肪组织中甘油三酯的组成。
The composition of triglycerides from liver, egg yolk and adipose tissue of the laying hen.
作者信息
Husbands D R
出版信息
Biochem J. 1970 Nov;120(2):365-71. doi: 10.1042/bj1200365.
The composition of the triglycerides of liver, egg yolk and adipose tissue of laying hens fed on a standard diet were investigated by using argentation thin-layer chromatography to separate the triglycerides according to their degree of unsaturation. About 40% of liver triglycerides consisted of one saturated and two monoenoic fatty acids. Triglycerides containing linoleate were more abundant in adipose tissue than in either yolk or liver. Hydrolysis by pancreatic lipase of the tissue triglycerides and fractions obtained from these triglycerides showed that the triglycerides of adipose tissue had a less ordered arrangement of fatty acids at the 2-position than did either yolk or liver triglycerides. The labelling patterns of triglycerides formed in liver slices incubated in the presence of [1-(3)(14)C]glycerol indicated that triglycerides containing four or more double bonds are formed to a greater extent than are other triglyceride fractions. This is evidence for the concept that the type of triglyceride formed depends on the availability of fatty acids to the liver cells.
通过银化薄层色谱法根据甘油三酯的不饱和度对其进行分离，研究了以标准日粮喂养的产蛋母鸡肝脏、蛋黄和脂肪组织中甘油三酯的组成。肝脏甘油三酯约40%由一种饱和脂肪酸和两种单烯脂肪酸组成。含亚油酸的甘油三酯在脂肪组织中比在蛋黄或肝脏中更为丰富。胰腺脂肪酶对组织甘油三酯及其分离得到的组分进行水解，结果表明，脂肪组织甘油三酯在2位脂肪酸的排列顺序比蛋黄或肝脏甘油三酯的无序程度更低。在[1-(3)(14)C]甘油存在下孵育的肝切片中形成的甘油三酯的标记模式表明，含四个或更多双键的甘油三酯的形成程度比其他甘油三酯组分更高。这为甘油三酯的形成类型取决于肝细胞可利用脂肪酸这一概念提供了证据。
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